To browse Academia. Sensitive assays are needed for detection of residual HIV in patients with undetectable plasma viral loads to determine if eradication strategies are effective. The gold standard quantitative viral outgrowth assay QVOA underestimates the magnitude of the viral reservoir, while sensitive PCR-based assays lack the ability to distinguish replication competent from defective virus. We sought to determine whether xenograft of leukocytes from HIV-1 infected patients with undetectable plasma viral loads into severely immunocompromised mice would result in viral amplification and measurable viral loads within the aberrant murine host.
Successful xenograft of mice was confirmed by flow cytometry. Human CD8' T cells were depleted in humanized mice with depleting antibody, and CD4' T cells were activated in a subset of mice with activating anti-CD3. We similarly detected SIV in macaquized mice by seven days post-xenograft.
Conclusions: The MVOA has the potential to serve as a powerful tool to identify residual HIV-1 in patients with undetectable viral loads, such as those who have undergone promising cure therapies. The gold standard quantitative viral outgrowth assay QVOA underestimates the magnitude of the viral reservoir.
We sought to determine whether xenograft of leukocytes from HIV-1 infected patients with undetectable plasma viral loads into immunocompromised mice would result in viral amplification. In some cases humanized mice were also treated with activating anti-CD3 antibody.
Acknowledging Australian Indigenous cultural diversity involves respecting local Indigenous knowledge and perspectives. This can be difficult for teachers who do not know about Indigenous people and their knowledge. The purpose was to consolidate findings to increase positive intercultural actions in the wider education community. An important aspect of the project is addressing gaps in western, non-Indigenous teacher kn Purpose-Four forms of school bullying behaviors among US adolescents and their association with socio-demographic characteristics, parental support and friends were examined.
