Cell Stabilisation Reagents for use in Flow Cytometry. Search for:. Select Date: All Dates Collapse all Expand all. Optimisation of storage conditions was further investigated to improve immunophenotyping after freezing.
Further studies will aim to demonstrate the upper limit of time the sample will remain stable and suitable for immunophenotyping. Experiments will also be conducted to examine the suitability for use in phosphoflow assays. View Document. Many central nervous system CNS diseases such as inflammatory neurologic diseases, leukaemia, or autoimmune diseases, are associated with the presence of white blood cells in the cerebral spinal fluid CSF.
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The detection of leukocyte subsets in CSF is of vital importance for the accurate diagnosis and treatment of such diseases. However, low cellularity and cell viability of leukocytes causes challenges when analysing CSF with flow cytometry, and it is recommended that native CSF samples are analysed within 1 hour of lumbar puncture Kraan et. This eliminates the possibility of multicentre studies and centralised diagnostics Sedek et al. Samples were tested by flow cytometry at different time points over 3 days.
Data showed that the TransFix treated samples provided consistent cell recovery for at least 72 hours after collection. This paper focuses on the validation of the Vycap method for isolation of individual CTC's. These were analyses downstream for DNA. The authors used TransFix blood collection tubes CTC-TVTs to allow VyCAP single CTC isolation for up to 48 h after blood draw and preserve cell morphology while the cells do still retain a certain flexibility that allows efficient separation of the excess leukocytes, while keeping the CTC in the pore at the bottom of the microwell.
Stevens, L. Oomens, J. Broekmaat, J. Weersink, F. Abali, J. Swennenhuis, A. Tibbe Cytometry Part A, 93 12 ; The protozoan parasite histomonas is causative agent of histomonosis in Turkeys and chickens and causes a high mortality rate in Turkeys. Experimental vaccination has shown to protect these species. TransFix was used to stabilise 0. Mitraa, W. Gernerb, F. Kidanea, P. Wernsdorfa, M. Hessa, A. Liebharta Vaccine 35 33 : Use of TransFix cerebrospinal fluid storage tubes prevents cellular loss and enhances flow cytometric detection of malignant hematological cells after 18 hours of storage.
