Official websites use. Share sensitive information only on official, secure websites. Gluteofemoral adipose tissue areas are known to be poorly metabolically reactive. Mechanical massage has previously been reported to show morphological and functional impact on this tissue. Total RNA was extracted from femoral adipose tissue biopsies for gene expression studies. Microdialysis was carried out in the femoral adipose tissue in order to assess lipolytic responsiveness via glycerol determination and changes in local blood flow following perfusion of a lipolytic agent, isoproterenol.
Evaluations were performed before and after the 6-week experimental period. Mechanical massage initiated important modifications in gene expression profile. The lipid-mobilizing effect of isoproterenol was enhanced after the experimental period. Basal local blood flow and isoproterenol-induced vasodilatation were also improved.
The protocol of mechanical massage used in the study promoted noticeable changes in the expression of genes involved in metabolic pathways. The lipolytic and local adipose tissue blood flow responses initiated by isoproterenol were significantly enhanced. Keywords: Gene expression profiling, Femoral adipose tissue, Isoproterenol-induced lipolysis, Microdialysis, Adipose tissue blood flow.
Body fat distribution is an important metabolic and cardiovascular risk factor. Several studies have revealed that obesity-related health risks depend on the accumulation of abdominal and visceral fat.
Studies on adipose tissue biology and physiology have shown that striking differences exist regarding fatty acid uptake mechanisms and lipolytic responsiveness between visceral and gluteofemoral fat. Differential adipose tissue blood flow responsiveness has also been reported between fat deposits. Visceral adipose tissue adipocytes are considered to be the smallest and the most responsive to lipid-mobilizing hormones while subcutaneous gluteofemoral adipose tissue GAT adipocytes are known to be less metabolically reactive [ 1 ].
